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KMID : 0364820130490010008
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Korean Journal of Microbiology
2013 Volume.49 No. 1 p.8 ~ p.16
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Isolation and Characterization of the gtfA Gene Encoding GAL4-Like Transcription Factor in Aspergillus nidulans
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Park Jae-Sin
Han Dong-Min
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Abstract
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A GAL4 type transcription factor gene (formally annotated as AN3912) locating downstream of sndA (AN3911) was
characterized. The putative transcription factor carries both Zn(II)2Cys6 binuclear cluster DNA-binding domain and
transcription activator domain. The gene named gtfA (gal4 type transcription factor) had an open reading frame
which consisted of 762 amino acids and was disrupted by three introns. The deletion mutant produced reduced
amount of conidia but increased amount of fruiting bodies, suggesting that the GtfA make function in decision of
asexual preferential to sexual development. The forced over expression of gtfA caused the retardation of fruiting
body formation on high glucose concentration. The transcript level of gtfA was kept constant through the life cycle
except late vegetative stage and early sexual development stage during which slight increase was found. The
expression of gtfA was not significantly affected by sexual or asexual development regulators, such as VeA, NsdD or
FluG, FadA, and SfaD. The GtfA repressed the nsdC transcription, which suggested that GftA control sexual
development negatively via negative regulation of nsdC expression.
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KEYWORD
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Aspergillus nidulans, development, Zn(II)2Cys6 transcription factor
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